Useful Links & Tools
- MSDS
- Specification Sheet
- Certificate of Analysis
- FT-IR Raman
- FT-NMR
- Bulk Quote
- Similar Products
D2438
Sigma
Dimethyl sulfoxide
Biotechnology Performance Certified, sterile-filtered, hybridoma tested, meets EP, USP testing specifications
| Synonym: | DMSO, Methyl sulfoxide |
| CAS Number: | 67-68-5 |
| Linear Formula: | (CH3)2SO |
| Molecular Weight: | 78.13 |
| Beilstein Registry Number: | 506008 |
| EC Number: | 200-664-3 |
| MDL number: | MFCD00002089 |
| PubChem Substance ID: | 24893688 |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions |
| Application | Human and animal cell lines grown in culture are generally stored frozen. Freezing protects the cell line from changes due to genetic drift and minimizes risk of contamination. Liquid nitrogen used in conjunction with a cryoprotective agent such as DMSO is a widely used method for preserving cells. Without the presence of a cryoprotective agent, freezing is lethal to most mammalian cells. Damage is caused by mechanical injury by ice crystals, concentration of electrolytes, pH changes, and denaturation of proteins. These lethal effects are minimized by adding a cryoprotective agent which lowers the freezing point and allows for a slower cooling rate. BPC-tested DMSO also meets the requirements of biotechnology and tissue engineering applications. |
| Caution | Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product. |
| Features and Benefits | Ready-to-use sterile filtered product conveniently packaged septum screw-capped amber bottles. Meets USP and EP testing requirements |
Properties
| grade | Biotechnology Performance Certified |
| vapor density | 2.7 (vs air) |
| vapor pressure | 0.42 mmHg ( 20 °C) |
| sterility | sterile-filtered |
| assay | (USP XXIV) |
| autoignition temp. | 573 °F |
| expl. lim. | 42 %± , 63 °F |
| refractive index | n |
| bp | 189 °C(lit.) |
| mp | 16-19 °C(lit.) |
| density | 1.10 g/mL(lit.) |
| suitability | hybridoma tested |
| meets EP, USP testing specifications |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US) |
| WGK Germany | 1 |
| RTECS | PV6210000 |
| UEL | 42.00%(V) |
| LEL | 3.50%(V) |
| Flash Point(F) | 188.6 °F |
| Flash Point(C) | 87 °C |
References
| Merck | Merck 13,3285 |
| Beilstein | Beil. 1,IV,1277 |
| Fieser | Fieser 1,296 / Fieser 2,157 / Fieser 3,119 / Fieser 4,192 / Fieser 5,263 / Fieser 6,225 / Fieser 7,133 / Fieser 8,198 / Fieser 9,184 / Fieser 9,189 / Fieser 10,166 / Fieser 11,214 / Fieser 12,212 / Fieser 13,124 / Fieser 16,149 |
| reference | Aldrich MSDS 1, 1308:D / Corp MSDS 1 (2), 2440:C / FT-IR 2 (1), 1492:B / FT-IR 1 (1), 879:A / FT-NMR 1 (1), 1417:A / IR-Spectra (3), 529:A / NMR-Reference 2 (2), 781:A / RegBook 1 (1), 1043:A / Sax 6, 1201 / Structure Index 1, 162:A:1 / Vapor Phase 3, 831:A |







