D2438

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D2438

Sigma

 

Dimethyl sulfoxide

Biotechnology Performance Certified, sterile-filtered, hybridoma tested, meets EP, USP testing specifications

Synonym:DMSO, Methyl sulfoxide
CAS Number:67-68-5
Linear Formula:(CH3)2SO
Molecular Weight:78.13
Beilstein Registry Number:506008
EC Number:200-664-3
MDL number:MFCD00002089
PubChem Substance ID:24893688

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Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
ApplicationHuman and animal cell lines grown in culture are generally stored frozen. Freezing protects the cell line from changes due to genetic drift and minimizes risk of contamination. Liquid nitrogen used in conjunction with a cryoprotective agent such as DMSO is a widely used method for preserving cells. Without the presence of a cryoprotective agent, freezing is lethal to most mammalian cells. Damage is caused by mechanical injury by ice crystals, concentration of electrolytes, pH changes, and denaturation of proteins. These lethal effects are minimized by adding a cryoprotective agent which lowers the freezing point and allows for a slower cooling rate. BPC-tested DMSO also meets the requirements of biotechnology and tissue engineering applications.
CautionSupercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
Features and BenefitsReady-to-use sterile filtered product conveniently packaged septum screw-capped amber bottles. Meets USP and EP testing requirements

Properties

gradeBiotechnology Performance Certified
vapor density2.7 (vs air)
vapor pressure0.42 mmHg ( 20 °C)
sterilitysterile-filtered
assay (USP XXIV)
autoignition temp.573 °F
expl. lim.42 %± , 63 °F
refractive indexn20/D 1.479(lit.)
bp189 °C(lit.)
mp16-19 °C(lit.)
density1.10 g/mL(lit.)
suitabilityhybridoma tested
 meets EP, USP testing specifications

Safety

Personal Protective EquipmentEyeshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US)
WGK Germany1
RTECSPV6210000
UEL42.00%(V)
LEL3.50%(V)
Flash Point(F)188.6 °F
Flash Point(C)87 °C

References

MerckMerck 13,3285
BeilsteinBeil. 1,IV,1277
FieserFieser 1,296 / Fieser 2,157 / Fieser 3,119 / Fieser 4,192 / Fieser 5,263 / Fieser 6,225 / Fieser 7,133 / Fieser 8,198 / Fieser 9,184 / Fieser 9,189 / Fieser 10,166 / Fieser 11,214 / Fieser 12,212 / Fieser 13,124 / Fieser 16,149
referenceAldrich MSDS 1, 1308:D / Corp MSDS 1 (2), 2440:C / FT-IR 2 (1), 1492:B / FT-IR 1 (1), 879:A / FT-NMR 1 (1), 1417:A / IR-Spectra (3), 529:A / NMR-Reference 2 (2), 781:A / RegBook 1 (1), 1043:A / Sax 6, 1201 / Structure Index 1, 162:A:1 / Vapor Phase 3, 831:A